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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.62 (2016) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/517
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| タイトル: | Differential effects of lysophosphatidylcholine and ACh on muscarinic K+,non-selective cation and Ca2+ currents in guinea-pig atrial cells |
| 著者: | Li, Libing
Matsuoka, Isao
Sakamoto, Kazuho
Kimura, Junko |
| 学内所属: | 薬理学講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 62 |
| 号: | 1 |
| 開始ページ: | 27 |
| 終了ページ: | 35 |
| 発行日: | 2016年 |
| 抄録: | We compared the effects of lysophosphatidylcholine (LPC) and acetylcholine (ACh) on IK(ACh), ICa and a non-selective cation current (INSC) in guinea-pig atrial myocytes to clarify whether LPC and ACh activate similar Gi/o-coupled effector systems. IK(ACh), ICa and INSC were analyzed in single atrial myocytes by the whole cell patch-clamp. LPC induced INSC in a concentration-dependent manner in atrial cells. ACh activated IK(ACh), but failed to evoke INSC. LPC also activated IK(ACh) but with significantly less potency than ACh. The effects of both ligands on IK(ACh) were inhibited by intracellular loading of pre-activated PTX. This treatment also inhibited LPC-induced INSC, indicating that IK(ACh) and INSC induced by LPC are both mediated by Gi/o. LPC and ACh had similar potencies in inhibiting ICa, which was pre-augmented by forskolin, indicating that LPC and ACh activate similar amounts of α-subunits of Gi/o. The different effects of LPC and ACh on IK(ACh) and INSC may suggest that LPC and ACh activate Gi/o having different types of βγ subunits, and that LPC-induced INSC may be mediated by βγ subunits of Gi/o, which are less effective in inducing IK(ACh). |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/517 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/517/1/FksmJMedSci_62_p27.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2015-23 |
| PubMed番号: | 26911304 |
| 関連ページ: | http://doi.org/10.5387/fms.2015-23 |
| 権利情報: | © 2016 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.62 (2016)
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