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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.68 (2022) >
Please use this identifier to cite or link to this item:
http://ir.fmu.ac.jp/dspace/handle/123456789/1929
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| Title: | Population analysis of oseltamivir-resistant variants for the rapid prediction of drug susceptibility by real-time reverse transcription polymerase chain reaction |
| Authors: | Sato, Masatoki
Hashimoto, Koichi
Hosoya, Mitsuaki |
| Affiliation: | 小児科学講座 |
| Source title: | Fukushima Journal of Medical Science |
| Volume: | 68 |
| Issue: | 3 |
| Start page: | 153 |
| End page: | 159 |
| Issue Date: | 2022 |
| Abstract: | This study investigated whether quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), using specific probes composed of locked nucleic acids (LNA/qRT-PCR), designed to evaluate H1N1 pdm09 H275Y, H3N2 E119V and R292K variant populations, could replace a neuraminidase (NA) inhibition assay to determine the 50% inhibitory concentration (IC50) of NA activity.For H1N1 pdm09, when the H275Y variant RNA load was 50% or 70% and the infective H275Y variant load was 40% or 70%, the IC50 were >10- and 100-fold higher, respectively, than that of the wild-type (WT) strain. For H3N2, when the E119V RNA load and infective E119V variant load were >90% and >60%, respectively, the IC50 of the mixed sample was >10-fold higher than that of the WT strain. The variant-mixed samples with a 70% or 80% R292K variant RNA load and a 60% or 70% infective R292K variant load exhibited >10- and 100-fold decreased susceptibility, respectively, compared with that of the WT. A positive correlation between the variant RNA load and infective variant load populations was observed.The LNA/qRT-PCR method can be used to improve the treatment and management of patients during antiviral therapy for influenza virus infection. |
| Publisher: | The Fukushima Society of Medical Science |
| Publisher (Alternative foam): | 福島医学会 |
| language: | eng |
| URI: | http://ir.fmu.ac.jp/dspace/handle/123456789/1929 |
| Full text URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/1929/1/FksmJMedSci_68_p153.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2022-15 |
| PubMed ID: | 36047170 |
| Related Page: | https://doi.org/10.5387/fms.2022-15 |
| Rights: | © 2022 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license. |
| Rights: | https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| Appears in Collections: | Vol.68 (2022)
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