DSpace Fukushima Medical University

福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.51 (2005) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/168

Files in This Item:

File Description SizeFormat
FksmJMedSci_51_p41.pdf1.19 MBAdobe PDFDownload
Title: Role of CpG ODN in concanavalin A-induced hepatitis in mice
Authors: Abe, Kazumichi
Ohira, Hiromasa
Kobayashi, Hiroko
Rai, Tsuyoshi
Saito, Hironobu
Takahashi, Atsushi
Sato, Yukio
Affiliation: 内科学第二講座
Source title: Fukushima Journal of Medical Science
Volume: 51
Issue: 1
Start page: 41
End page: 49
Issue Date: Jun-2005
Abstract: OBJECTIVE: To investigate the effects of an intradermal injection of oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs on concanavalin A (Con A)-induced hepatitis, an experimental model of immune-mediated hepatitis. METHODS: Con A was injected intravenously into Balb/c mice. Twelve hours after Con A challenge, blood and liver samples were obtained. CpG ODN was injected intradermally 48 hours before Con A challenge. The extent of liver injury was assessed by determining serum alanine transaminase (ALT) and by liver histology. Serum levels of cytokines, including interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-4 and IL-5, were measured by enzyme-linked immunosorbent assay. RESULTS: Co-administration of Con A and CpG ODN significantly increased serum ALT in mice compared with that in the case of administration of Con A alone (10,268 +/- 4,654 and 1,140 +/- 832 IU/1, respectively, p<0.05). In liver histology, mice treated with CpG ODN and Con A showed more extensive midzonal necrosis than did mice treated with Con A alone. These mice also showed significant increases in serum TNF-alpha and IFN-gamma and decrease in serum IL-5. CONCLUSIONS: The results indicate that CpG ODNs aggravate Con A-induced hepatitis by stimulating the production of T-helper-1 (Th1) cytokines, TNF-alpha and IFN-gamma, suggesting that bacterial DNA that contains unmethylated CpG motifs may contribute to the exacerbation of immune-mediated liver injury.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/168
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/168/1/FksmJMedSci_51_p41.pdf
ISSN: 0016-2590
PubMed ID: 16167672
Rights: © 2005 The Fukushima Society of Medical Science
Appears in Collections:Vol.51 (2005)

Files in This Item:

File Description SizeFormat
FksmJMedSci_51_p41.pdf1.19 MBAdobe PDFDownload

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard