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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.51 (2005) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/168
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| タイトル: | Role of CpG ODN in concanavalin A-induced hepatitis in mice |
| 著者: | Abe, Kazumichi
Ohira, Hiromasa
Kobayashi, Hiroko
Rai, Tsuyoshi
Saito, Hironobu
Takahashi, Atsushi
Sato, Yukio |
| 学内所属: | 内科学第二講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 51 |
| 号: | 1 |
| 開始ページ: | 41 |
| 終了ページ: | 49 |
| 発行日: | 2005年6月 |
| 抄録: | OBJECTIVE: To investigate the effects of an intradermal injection of oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs on concanavalin A (Con A)-induced hepatitis, an experimental model of immune-mediated hepatitis. METHODS: Con A was injected intravenously into Balb/c mice. Twelve hours after Con A challenge, blood and liver samples were obtained. CpG ODN was injected intradermally 48 hours before Con A challenge. The extent of liver injury was assessed by determining serum alanine transaminase (ALT) and by liver histology. Serum levels of cytokines, including interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-4 and IL-5, were measured by enzyme-linked immunosorbent assay. RESULTS: Co-administration of Con A and CpG ODN significantly increased serum ALT in mice compared with that in the case of administration of Con A alone (10,268 +/- 4,654 and 1,140 +/- 832 IU/1, respectively, p<0.05). In liver histology, mice treated with CpG ODN and Con A showed more extensive midzonal necrosis than did mice treated with Con A alone. These mice also showed significant increases in serum TNF-alpha and IFN-gamma and decrease in serum IL-5. CONCLUSIONS: The results indicate that CpG ODNs aggravate Con A-induced hepatitis by stimulating the production of T-helper-1 (Th1) cytokines, TNF-alpha and IFN-gamma, suggesting that bacterial DNA that contains unmethylated CpG motifs may contribute to the exacerbation of immune-mediated liver injury. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/168 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/168/1/FksmJMedSci_51_p41.pdf |
| ISSN: | 0016-2590 |
| DOI: | 10.5387/fms.51.41 |
| PubMed番号: | 16167672 |
| 関連ページ: | https://doi.org/10.5387/fms.51.41 |
| 権利情報: | © 2005 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.51 (2005)
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