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Vol.51 (2005) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/166

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Title: Effectiveness of intragastric immunization with protein and oligodeoxynucleotides containing a CpG motif for inducing a gastrointestinal mucosal immune response in mice
Other Titles: Mucosal immunization with protein and CpG-DNA
Authors: Hikichi, Takuto
Kobayashi, Hiroko
Oyama, Hitoshi
Yamamoto, Go
Watanabe, Hiroshi
Irisawa, Atsushi
Obara, Katsutoshi
Sato, Yukio
Affiliation: 内科学第二講座
Source title: Fukushima Journal of Medical Science
Volume: 51
Issue: 1
Start page: 19
End page: 31
Issue Date: Jun-2005
Abstract: PURPOSE: To investigate a new modality of mucosal vaccines, we evaluated the effectiveness of intragastric immunization for inducing a mucosal immune response in the gastrointestinal tract. METHODS: Mice were immunized with beta-galactosidase (beta-gal) and synthesized oligodeoxynucleotides containing a CpG motif (CpG-DNA) by intragastric injection, and the immune response was compared with those induced by 3 other immunization forms: intranasal, oral, and intradermal. RESULTS: Intragastric immunization with beta-gal and CpG-DNA induced significant anti-beta-gal fecal IgA production at 2 weeks; however, at 4 weeks the response was lacking. In contrast, intranasal immunization with beta-gal and CpG-DNA induced the highest anti-beta-gal fecal IgA production at 4 weeks. CONCLUSION: Although intragastric immunization with protein and CpG-DNA induces a mucosal immune response in the gastrointestinal tract, intranasal immunization is the most effective to induce both mucosal and systemic immune responses. This finding may increase the possibility for developing vaccines against mucosal pathogens, especially Helicobacter pylori.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/166
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/166/1/FksmJMedSci_51_p19.pdf
ISSN: 0016-2590
PubMed ID: 16167670
Rights: © 2005 The Fukushima Society of Medical Science
Appears in Collections:Vol.51 (2005)

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