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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.67 (2021) >
Please use this identifier to cite or link to this item:
http://ir.fmu.ac.jp/dspace/handle/123456789/1380
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| Title: | Comprehensive analysis of gene expression of isolated pancreatic islets during pretransplant culture |
| Authors: | Haga, Junichiro
Sato, Naoya
Anazawa, Takayuki
Kimura, Takashi
Kenjo, Akira
Gotoh, Mitsukazu
Marubashi, Shigeru |
| Affiliation: | 肝胆膵・移植外科学講座 |
| Source title: | Fukushima Journal of Medical Science |
| Volume: | 67 |
| Issue: | 1 |
| Start page: | 17 |
| End page: | 26 |
| Issue Date: | 2021 |
| Abstract: | Background: The aim of this study was to investigate the effect of pretransplant culture on the survival of panThe aim of this study was to investigate the effect of pretransplant culture on the survival of pancreatic islet grafts, and to determine the biological characteristics of isolated islets during pretransplant culture. Methods: The survival of islets from Wistar rats, transplanted to diabetic C57BL/B6 mice, was compared between fresh islets and cultured islets. A comprehensive gene expression analysis was employed to investigate biological processes during pretransplant culture, and in vitro validation studies were performed. Results: Survival of cultured xenografts was significantly prolonged as compared to that of fresh islets (fresh:12.5 ± 1.9 days, 1-day cultured:16.0 ± 1.3 days (p= 0.017), 3-day cultured:17.0 ± 2.6 days (p= 0.014)). Comprehensive gene expression analysis identified significant upregulation of annotated functions associated with inflammation in cultured groups. Six proinflammatory genes, including heme oxygenase 1 (HO-1) and IL-6, were significantly upregulated during culture. Validation studies revealed significantly higher levels of IL-6 in the supernatant of cultured islets and HO-1 in the cultured islets when compared with fresh islets. Conclusion: Transplantation of cultured islets induced significant but minimal prolongation of graft survival in xenogeneic combinations. Comprehensive analysis of gene expression in cultured islets showed biological processes associated with proinflammation during culture. |
| Publisher: | The Fukushima Society of Medical Science |
| Publisher (Alternative foam): | 福島医学会 |
| language: | eng |
| URI: | http://ir.fmu.ac.jp/dspace/handle/123456789/1380 |
| Full text URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/1380/1/FksmJMedSci_67_p17.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2020-25 |
| PubMed ID: | 33597316 |
| Related Page: | https://doi.org/10.5387/fms.2020-25 |
| Rights: | © 2021 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license. |
| Rights: | https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| Appears in Collections: | Vol.67 (2021)
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