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Vol.64 (2018) >

このアイテムの引用には次の識別子を使用してください: http://ir.fmu.ac.jp/dspace/handle/123456789/674


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タイトル: Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process
著者: Asai, Emiko
Yamamoto, Masaya
Ueda, Kazuki
Waguri, Satoshi
学内所属: 形成外科学講座
誌名/書名: Fukushima Journal of Medical Science
巻: 64
号: 1
開始ページ: 15
終了ページ: 22
発行日: 2018年
抄録: To investigate the possible implications of autophagy, one of the degradation pathways induced by metabolic stress, in the dynamic reconstructive process of wound healing, the appearance and changes of punctate structures for microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, were examined in a rat skin wound healing model. Although the ratio of LC3-II/LC3-I in Western blotting was not evidently changed during the wound healing process, LC3-positive dots were clearly observed in fibroblasts and myofibroblasts, and occasionally in macrophages, by immunohistofluorescence microscopy. Some of the LC3-positive dots were colocalized with Atg16L signal, an isolation membrane marker, and electron microscopy revealed the presence of typical autophagosomes in fibroblasts near the margin of the wound. The number of LC3-positive dots per fibroblast increased during the later period of the proliferation phase, and interestingly, it was higher in the margin than the center of the wound. It was also high in the periwound skin area. These results suggest that drastic functional changes in fibroblasts during wound healing process are accompanied by the alteration of the autophagy-lysosomal degradation system.
出版者: The Fukushima Society of Medical Science
出版者(異表記): 福島医学会
本文の言語: eng
このページのURI: http://ir.fmu.ac.jp/dspace/handle/123456789/674
本文URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/674/1/FksmJMedSci_64_p15.pdf
ISSN: 0016-2590
DOI: 10.5387/fms.2016-13
PubMed番号: 29343655
関連ページ: https://doi.org/10.5387/fms.2016-13
権利情報: © 2018 The Fukushima Society of Medical Science
出現コレクション:Vol.64 (2018)


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FksmJMedSci_64_p15.pdf6.3 MBAdobe PDFダウンロード



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