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Vol.64 (2018) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/674

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Title: Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process
Authors: Asai, Emiko
Yamamoto, Masaya
Ueda, Kazuki
Waguri, Satoshi
Affiliation: 形成外科学講座
Source title: Fukushima Journal of Medical Science
Volume: 64
Issue: 1
Start page: 15
End page: 22
Issue Date: 2018
Abstract: To investigate the possible implications of autophagy, one of the degradation pathways induced by metabolic stress, in the dynamic reconstructive process of wound healing, the appearance and changes of punctate structures for microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, were examined in a rat skin wound healing model. Although the ratio of LC3-II/LC3-I in Western blotting was not evidently changed during the wound healing process, LC3-positive dots were clearly observed in fibroblasts and myofibroblasts, and occasionally in macrophages, by immunohistofluorescence microscopy. Some of the LC3-positive dots were colocalized with Atg16L signal, an isolation membrane marker, and electron microscopy revealed the presence of typical autophagosomes in fibroblasts near the margin of the wound. The number of LC3-positive dots per fibroblast increased during the later period of the proliferation phase, and interestingly, it was higher in the margin than the center of the wound. It was also high in the periwound skin area. These results suggest that drastic functional changes in fibroblasts during wound healing process are accompanied by the alteration of the autophagy-lysosomal degradation system.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/674
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/674/1/FksmJMedSci_64_p15.pdf
ISSN: 0016-2590
DOI: 10.5387/fms.2016-13
PubMed ID: 29343655
Related Page: https://doi.org/10.5387/fms.2016-13
Rights: © 2018 The Fukushima Society of Medical Science
Appears in Collections:Vol.64 (2018)

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FksmJMedSci_64_p15.pdf6.3 MBAdobe PDFDownload

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