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Vol.63 (2017) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/642

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Title: Modification of the HCMV-specific IFN-γ release test (QuantiFERON-CMV) and a novel proposal for its application
Authors: Kobayashi, Takahiro
Sato, Jun-Ichi
Ikuta, Kazufumi
Kanno, Ryoko
Nishiyama, Kyoko
Koshizuka, Tetsuo
Ishioka, Ken
Suzutani, Tatsuo
Affiliation: 微生物学講座
Source title: Fukushima Journal of Medical Science
Volume: 63
Issue: 2
Start page: 64
End page: 74
Issue Date: 2017
Abstract: Human cytomegalovirus (HCMV) is universally distributed among humans without any adverse effects; however, it induces severe diseases in immunocompromised patients such as organ transplant recipients and AIDS patients. To manage these immunocompromised patients, an easy clinical examination for the monitoring of disease risk is required. In this study, we modified the interferon-γ (IFN-γ) release test (QuantiFERON®-CMV) using HCMV immediate early-1 (IE-1) or pp65 whole proteins, or UV-inactivated HCMV particles as an antigen. The response of heparinized peripheral blood from healthy volunteers to the pp65 protein showed an obvious dose-dependent sigmoid curve, although no correlation was observed between results of this assay and an ELISPOT assay. The addition of pp65 to the blood samples at a final concentration of 1×10^3 to 1×10^5 pg/ml was found to be optimum. Using this assay, we observed a significant enhancement in cellular immunity in volunteers after the daily ingestion of yogurt for 8 weeks, which suggested a novel application of the assay in addition to monitoring HCMV infection risk. IFN-γ secretion from peripheral blood cells on HCMV-antigen stimulation differed significantly between individuals; therefore, the assay could not be normalized. Nevertheless, it was found to be particularly useful for observing fluctuations in cellular immune activity on an individual level.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/642
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/642/1/FksmJMedSci_63_p64.pdf
ISSN: 0016-2590
DOI: 10.5387/fms.2017-01
PubMed ID: 28638004
Related Page: http://doi.org/10.5387/fms.2017-01
Rights: © 2017 The Fukushima Society of Medical Science
Appears in Collections:Vol.63 (2017)

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FksmJMedSci_63_p64.pdf2.21 MBAdobe PDFDownload

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