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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.62 (2016) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/518
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| タイトル: | Protection from lethal herpes simplex virus type 1 infection by vaccination with a UL41-deficient recombinant strain |
| 著者: | Koshizuka, Tetsuo
Ishioka, Ken
Kobayashi, Takahiro
Ikuta, Kazufumi
Suzutani, Tatsuo |
| 学内所属: | 微生物学講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 62 |
| 号: | 1 |
| 開始ページ: | 36 |
| 終了ページ: | 42 |
| 発行日: | 2016年 |
| 抄録: | The UL41 gene of herpes simplex virus type 1 (HSV-1) encodes a virion host shut off protein which is involved in immune evasion. The growth and virulence of HSV-1 is markedly reduced by the deletion of UL41. In this report, the UL41-deleted recombinant HSV-1 strain VR∆41 was evaluated as a prophylactic live attenuated vaccine against lethal HSV-1 infection in a mouse model. Intraperitoneal (i.p.) inoculation with the VR∆41 strain clearly inhibited lethal wild-type HSV-1 (VR-3 strain) infection after both i.p. and intracerebral (i.c.) inoculations. Vaccination with the VR∆41 strain was safer than VR-3 vaccination and was able to protect against a wild-type challenge to the same degree as VR-3 vaccination. In contrast, i.p. inoculation with ultraviolet-irradiated VR-3 induced resistance against i.p. infection, but not against i.c. Although replication of the VR∆41 strain in mice was greatly reduced compared to that of the VR-3 strain, VR∆41 strain maintained the ability to spread to the central nervous system (CNS) from a peripheral inoculation site. These results indicated that the VR∆41 strain evoked a potent immune reaction through viral protein expression within CNS without the induction of lethal encephalitis. The entry of antigens into the CNS was essential for the establishment of protective immunity against the lethal HSV encephalitis. We concluded that only a live attenuated vaccine is able to afford a prophylactic effect against CNS infection with HSV. In order to fulfill this requirement, UL41-deleted viruses provide a strong candidate for use as a recombinant live vaccine. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/518 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/518/1/FksmJMedSci_62_p36.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2015-24 |
| PubMed番号: | 26983589 |
| 関連ページ: | http://doi.org/10.5387/fms.2015-24 |
| 権利情報: | © 2016 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.62 (2016)
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