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Vol.62 (2016) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/516

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Title: Swelling-activated and arachidonic acid-induced currents are TREK-1 in rat bladder smooth muscle cells
Authors: Fukasaku, Mitsuko
Kimura, Junko
Yamaguchi, Osamu
Affiliation: 泌尿器科学講座
薬理学講座
Source title: Fukushima Journal of Medical Science
Volume: 62
Issue: 1
Start page: 18
End page: 26
Issue Date: 2016
Abstract: Using the perforated patch voltage clamp, we investigated swelling-activated ionic channels (SACs) in rat urinary bladder smooth muscle cells. Hypo-osmotic (60%) bath solution increased a membrane current which was inhibited by the SAC inhibitor, gadolinium. The reversal potential of the hypotonicity-induced current shifted in the positive direction by increasing external K+ concentration. The hypotonicity-induced current was inhibited by extracellular acidic pH, phorbol ester and forskolin. These pharmacological properties are identical to those of arachidonic acid-induced current present in these cells, suggesting the presence of TREK-1, a four-transmembrane two pore domain K+ channel. Using RT-PCR we screened rat bladder smooth muscles and cerebellum for expression of TREK-1, TREK-2 and TRAAK mRNAs. Only TREK-1 mRNA was expressed in the bladder, while all three were expressed in the cerebellum. We conclude that a mechanosensitive K+ channel is present in rat bladder myocytes, which is activated by arachidonic acid and most likely is TREK-1. This K+ channel may have an important role in the regulation of bladder smooth muscle tone during urine storage.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/516
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/516/1/FksmJMedSci_62_p18.pdf
ISSN: 0016-2590
2185-4610
DOI: 10.5387/fms.2015-20
PubMed ID: 26911303
Related Page: http://doi.org/10.5387/fms.2015-20
Rights: © 2016 The Fukushima Society of Medical Science
Appears in Collections:Vol.62 (2016)

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FksmJMedSci_62_p18.pdf736.43 kBAdobe PDFDownload

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