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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.62 (2016) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/516
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| タイトル: | Swelling-activated and arachidonic acid-induced currents are TREK-1 in rat bladder smooth muscle cells |
| 著者: | Fukasaku, Mitsuko
Kimura, Junko
Yamaguchi, Osamu |
| 学内所属: | 泌尿器科学講座
薬理学講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 62 |
| 号: | 1 |
| 開始ページ: | 18 |
| 終了ページ: | 26 |
| 発行日: | 2016年 |
| 抄録: | Using the perforated patch voltage clamp, we investigated swelling-activated ionic channels (SACs) in rat urinary bladder smooth muscle cells. Hypo-osmotic (60%) bath solution increased a membrane current which was inhibited by the SAC inhibitor, gadolinium. The reversal potential of the hypotonicity-induced current shifted in the positive direction by increasing external K+ concentration. The hypotonicity-induced current was inhibited by extracellular acidic pH, phorbol ester and forskolin. These pharmacological properties are identical to those of arachidonic acid-induced current present in these cells, suggesting the presence of TREK-1, a four-transmembrane two pore domain K+ channel. Using RT-PCR we screened rat bladder smooth muscles and cerebellum for expression of TREK-1, TREK-2 and TRAAK mRNAs. Only TREK-1 mRNA was expressed in the bladder, while all three were expressed in the cerebellum. We conclude that a mechanosensitive K+ channel is present in rat bladder myocytes, which is activated by arachidonic acid and most likely is TREK-1. This K+ channel may have an important role in the regulation of bladder smooth muscle tone during urine storage. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/516 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/516/1/FksmJMedSci_62_p18.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2015-20 |
| PubMed番号: | 26911303 |
| 関連ページ: | http://doi.org/10.5387/fms.2015-20 |
| 権利情報: | © 2016 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.62 (2016)
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