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Vol.60 (2014) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/399

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Title: The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells
Authors: Suganami, Azusa
Sakamoto, Kazuho
Ono, Tomoyuki
Watanabe, Hirotake
Hijioka, Nahoko
Murakawa, Masahiro
Kimura, Junko
Affiliation: 麻酔科学講座
薬理学講座
Source title: Fukushima Journal of Medical Science
Volume: 60
Issue: 1
Start page: 22
End page: 30
Issue Date: 8-Aug-2014
Abstract: Shakuyakukanzoto (shao-yao-gan-cao-tang) is a commonly used Chinese traditional herbal medicine for the treatment of acute pain with muscle cramp. However, its mechanism of action is unclear. We previously reported that a low concentration of Kanzo (licorice) and isoliquiritigenin, a component of licorice, inhibited the potassium (K^+) current in H9c2 cells. Therefore, in the present study, we examined the effects of Shakuyakukanzoto, Shakuyaku or Kanzo on the K^+ current (IKur) in H9c2 cells. Shakuyakukanzoto inhibited IKur in a concentration-dependent manner. The half-maximal concentration of Shakuyakukanzoto was approximately 1.3 mg/mL and the Hill coefficient was 1.2. The order of potency of inhibiting IKur was Kanzo>Shakuyakukanzoto>Shakuyaku. Glycyrrhizin, a major component of licorice, had no inhibitory effect on IKur. A small interfering RNA experiment indicated that IKur was most likely to be Kv2.1 in H9c2 cells. Our results suggest that Shakuyakukanzoto may normalize intracellular and extracellular K^+ balance by inhibiting IKur and reducing K^+ efflux, while the Na^+-K^+ pump promotes K^+ influx into myofibers. Consequently, excess K^+ may be reduced from external space of myofibers. This may be a part of the Shakuyakukanzoto mechanism for improving muscle pain.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/399
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/399/1/FksmJMedSci_60_p22.pdf
ISSN: 0016-2590
2185-4610
DOI: 10.5387/fms.2013-16
PubMed ID: 24670676
Related Page: http://dx.doi.org/10.5387/fms.2013-16
Rights: © 2014 The Fukushima Society of Medical Science
Appears in Collections:Vol.60 (2014)

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