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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.60 (2014) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/399
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| タイトル: | The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells |
| 著者: | Suganami, Azusa
Sakamoto, Kazuho
Ono, Tomoyuki
Watanabe, Hirotake
Hijioka, Nahoko
Murakawa, Masahiro
Kimura, Junko |
| 学内所属: | 麻酔科学講座
薬理学講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 60 |
| 号: | 1 |
| 開始ページ: | 22 |
| 終了ページ: | 30 |
| 発行日: | 2014年8月8日 |
| 抄録: | Shakuyakukanzoto (shao-yao-gan-cao-tang) is a commonly used Chinese traditional herbal medicine for the treatment of acute pain with muscle cramp. However, its mechanism of action is unclear. We previously reported that a low concentration of Kanzo (licorice) and isoliquiritigenin, a component of licorice, inhibited the potassium (K^+) current in H9c2 cells. Therefore, in the present study, we examined the effects of Shakuyakukanzoto, Shakuyaku or Kanzo on the K^+ current (IKur) in H9c2 cells. Shakuyakukanzoto inhibited IKur in a concentration-dependent manner. The half-maximal concentration of Shakuyakukanzoto was approximately 1.3 mg/mL and the Hill coefficient was 1.2. The order of potency of inhibiting IKur was Kanzo>Shakuyakukanzoto>Shakuyaku. Glycyrrhizin, a major component of licorice, had no inhibitory effect on IKur. A small interfering RNA experiment indicated that IKur was most likely to be Kv2.1 in H9c2 cells. Our results suggest that Shakuyakukanzoto may normalize intracellular and extracellular K^+ balance by inhibiting IKur and reducing K^+ efflux, while the Na^+-K^+ pump promotes K^+ influx into myofibers. Consequently, excess K^+ may be reduced from external space of myofibers. This may be a part of the Shakuyakukanzoto mechanism for improving muscle pain. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/399 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/399/1/FksmJMedSci_60_p22.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2013-16 |
| PubMed番号: | 24670676 |
| 関連ページ: | http://dx.doi.org/10.5387/fms.2013-16 |
| 権利情報: | © 2014 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.60 (2014)
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