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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.55 (2009) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/234
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| タイトル: | Synthetic oligodeoxynucleotides induce gastritis in mice |
| その他のタイトル: | Gastritis induced by synthetic DNA |
| 著者: | Yamamoto, Go
Kobayashi, Hiroko
Hikichi, Takuto
Irisawa, Atsushi
Obara, Katsutoshi
Ohira, Hiromasa |
| 学内所属: | 内科学第二講座
内視鏡診療部 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 55 |
| 号: | 1 |
| 開始ページ: | 23 |
| 終了ページ: | 31 |
| 発行日: | 2009年6月 |
| 抄録: | To investigate whether DNA directly induces gastritis and/or peptic ulcer, we injected synthetic DNA including CpG motif (CpG-DNA) to mouse stomach. BALB/c mice were injected with either saline, acetic acid (AA), CpG-DNA, or Control-DNA. Mice were sacrificed, and sections of the stomachs were stained with hematoxylin and eosin. The lesions were histopathologically scored from 0 to 4 based on the extent of the inflammation. Populations of neutrophils and mononuclear cells infiltrated to the lesion were calculated. IFN-y mRNA expression at the injection site was analyzed by RT-PCR. The number of CpG motifs included in the complete genomes of H. pylori HP26695 and J99, Escherichia coli O157, and Salmonella Typhi was determined by genomic analysis of these bacteria. Intragastric injection with CpG-DNA induced gastritis, and statistical analysis of histological scores revealed a significant difference between saline vs CpG-DNA (p = 0.037). The population of mononuclear cells infiltrated to the lesions was significantly higher in mice injected with CpG-DNA than that injected with AA (p = 0.0061). IFN-gamma mRNA expression was detected in the CpG-DNA group. While H. pylori includes multiple CpG motifs in its genome, it has fewer than the other pathogenic gram-negative bacilli. We conclude that synthetic DNA including CpG motif directly causes gastritis in mice and induces IFN-gamma production in the stomach. Bacterial DNA including CpG motif is known to stimulate innate immunity and to cause inflammation. Thus, H. pylori genomic DNA may be one of the virulent factors involved in H. pylori infection. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/234 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/234/1/FksmJMedSci_55_p23.pdf |
| ISSN: | 0016-2590 |
| DOI: | 10.5387/fms.55.23 |
| PubMed番号: | 19999166 |
| 関連ページ: | https://doi.org/10.5387/fms.55.23 |
| 権利情報: | © 2009 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.55 (2009)
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