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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.54 (2008) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/221
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| タイトル: | Traffic of infused bone marrow cells after genetically-labeled syngeneic bone marrow transplantation following lethal irradiation in mice |
| その他のタイトル: | Traffic of infused BM cells after syngeneic BMT |
| 著者: | Satoh, Atai
Saito, Takuro
Sato, Yoshihiro
Tsuchiya, Takao
Kenjo, Akira
Kimura, Takashi
Kanno, Ryuzo
Suzuki, Hiroyuki
Kogure, Michio
Hoshino, Yutaka
Gotoh, Mitsukazu |
| 学内所属: | 外科学第一講座 |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 54 |
| 号: | 1 |
| 開始ページ: | 11 |
| 終了ページ: | 24 |
| 発行日: | 2008年6月 |
| 抄録: | Bone marrow (BM) cells are considered the source of stem cells for various organs. However, how quickly BM cells can penetrate and constitute lymphoid organs remains elusive. In the present study, we addressed this issue in a model using genetically-labeled syngeneic BM transplantation (BMT). METHODS: Donor BM cells were obtained from "green mice", transgenic mice with enhanced GFP. Lethally irradiated C57BL/6 mice were infused with 1 x 10(6) BM cells from the green mice through the tail vein. BM chimerism was analyzed by FACS and the presence of donor BM cells in thoracoabdominal organs was assessed by fluorescence microscopy. The commitment of BM cells was examined by immunohistochemical staining using epithelium-, macrophage-, B and T-lymphocyte, and endothelium-specific antibodies. RESULTS: BM chimerism reached 40+/- 18.5%, 82.6 +/- 23.4%, and 72 +/- 18% (mean +/- SD) at 1, 4, and 12 wks after BMT, respectively. GFP-positive cells were detected in all organs in the course of chimeric formation. Most GFP-positive cells were T and B lymphocytes in lymphoid systems including spleen, thymus, mesenteric lymph nodes and microvilli, and some were positive for macrophage and endothelial cell markers. CONCLUSIONS: Our results indicate that BM-derived cells migrate rapidly into various thoracoabdominal organs after BMT, and that lymphoid tissues are predominantly replaced with infused BM in lethally-irradiated mice. This confirmed the previous finding by others and suggests high interest of this model for further studies to characterize kinetics and roles of infused cells. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/221 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/221/1/FksmJMedSci_54_p11.pdf |
| ISSN: | 0016-2590 |
| DOI: | 10.5387/fms.54.11 |
| PubMed番号: | 18924549 |
| 関連ページ: | https://doi.org/10.5387/fms.54.11 |
| 権利情報: | © 2008 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.54 (2008)
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