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Vol.54 (2008) >

Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/221

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Title: Traffic of infused bone marrow cells after genetically-labeled syngeneic bone marrow transplantation following lethal irradiation in mice
Other Titles: Traffic of infused BM cells after syngeneic BMT
Authors: Satoh, Atai
Saito, Takuro
Sato, Yoshihiro
Tsuchiya, Takao
Kenjo, Akira
Kimura, Takashi
Kanno, Ryuzo
Suzuki, Hiroyuki
Kogure, Michio
Hoshino, Yutaka
Gotoh, Mitsukazu
Affiliation: 外科学第一講座
Source title: Fukushima Journal of Medical Science
Volume: 54
Issue: 1
Start page: 11
End page: 24
Issue Date: Jun-2008
Abstract: Bone marrow (BM) cells are considered the source of stem cells for various organs. However, how quickly BM cells can penetrate and constitute lymphoid organs remains elusive. In the present study, we addressed this issue in a model using genetically-labeled syngeneic BM transplantation (BMT). METHODS: Donor BM cells were obtained from "green mice", transgenic mice with enhanced GFP. Lethally irradiated C57BL/6 mice were infused with 1 x 10(6) BM cells from the green mice through the tail vein. BM chimerism was analyzed by FACS and the presence of donor BM cells in thoracoabdominal organs was assessed by fluorescence microscopy. The commitment of BM cells was examined by immunohistochemical staining using epithelium-, macrophage-, B and T-lymphocyte, and endothelium-specific antibodies. RESULTS: BM chimerism reached 40+/- 18.5%, 82.6 +/- 23.4%, and 72 +/- 18% (mean +/- SD) at 1, 4, and 12 wks after BMT, respectively. GFP-positive cells were detected in all organs in the course of chimeric formation. Most GFP-positive cells were T and B lymphocytes in lymphoid systems including spleen, thymus, mesenteric lymph nodes and microvilli, and some were positive for macrophage and endothelial cell markers. CONCLUSIONS: Our results indicate that BM-derived cells migrate rapidly into various thoracoabdominal organs after BMT, and that lymphoid tissues are predominantly replaced with infused BM in lethally-irradiated mice. This confirmed the previous finding by others and suggests high interest of this model for further studies to characterize kinetics and roles of infused cells.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/221
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/221/1/FksmJMedSci_54_p11.pdf
ISSN: 0016-2590
PubMed ID: 18924549
Rights: © 2008 The Fukushima Society of Medical Science
Appears in Collections:Vol.54 (2008)

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