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Please use this identifier to cite or link to this item: http://ir.fmu.ac.jp/dspace/handle/123456789/14

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Title: Culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection using aerobic culture
Other Titles: Culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection with aerobic culture
Time-dependence for sampling and detection of bacteria in platelets
Authors: Ezuki, Shoji
Kawabata, Kinuyo
Kanno, Takahiro
Ohto, Hitoshi
Affiliation: 輸血・移植免疫部
Source title: Transfusion
Volume: 47
Issue: 11
Start page: 2044
End page: 2049
Issue Date: Nov-2007
Abstract: BACKGROUND: Bacterial contamination of platelet products (PLTs) occurs at low concentrations requiring a period of incubation for growth to minimize sampling error. Culture-based detection methods also need sufficient incubation time; together these periods may limit the useful life of PLTs. This study characterizes the impact of sampling and detection times using two commercially-available bacteria detection products. STUDY DESIGN AND METHODS: Apheresis PLTs inoculated with nine bacterial species at low concentrations were sampled immediately, and after 24 hours following inoculation. Test results were analyzed following incubation at 16, 20 and 24 hours after sampling using two bacterial detection systems. RESULTS: When sampled immediately after inoculation, BacT/ALERT (BioMerieux) and Pall eBDS (Pall Corporation) failed to detect some PLTs inoculated with S. epidermidis, S. liquefaciens or P. aeruginosa and S. epidermidis, S. liquefaciens, B. cereus or P. aeruginosa, respectively. The BacT/ALERT was better at 20 hours (p<0.02), but not at 16 or 24 hours for time 0 sampling. When sampling occurred 24 hours after inoculation, there were no difference between the two systems. CONCLUSION: Results suggest that for either bacteria detection system, holding PLTs for 24 hrs prior to sampling improves the detection sensitivity for PLTs contaminated with low concentrations of bacteria and longer incubation periods improve detection.
Description: Title of published version: Culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection with aerobic culture
Publisher: Blackwell Publishing Inc
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/14
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/14/1/Transfusion_47_2044.pdf
ISSN: 0041-1132
DOI: 10.1111/j.1537-2995.2007.01428.x
PubMed ID: 17958533
Other version: http://dx.doi.org/10.1111/j.1537-2995.2007.01428.x
Rights: © 2007 American Association of Blood Banks. The definitive version is available at www.blackwell-synergy.com
Appears in Collections:a10 学術雑誌論文等 = Journal Article

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