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Vol.66 (2020) >

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Title: Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma
Authors: Kariya, Yoshinobu
Oyama, Midori
Ohtsuka, Mikio
Kikuchi, Nobuyuki
Hashimoto, Yasuhiro
Yamamoto, Toshiyuki
Affiliation: 生化学講座
皮膚科学講座
Source title: Fukushima Journal of Medical Science
Volume: 66
Issue: 3
Start page: 119
End page: 123
Issue Date: 2020
Abstract: α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/1354
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/1354/1/FksmJMedSci_66_p119.pdf
ISSN: 0016-2590
2185-4610
DOI: 10.5387/fms.2020-12
PubMed ID: 32779579
Related Page: https://doi.org/10.5387/fms.2020-12
Rights: © 2020 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license.
Rights: https://creativecommons.org/licenses/by-nc-sa/4.0/
Appears in Collections:Vol.66 (2020)

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