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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.66 (2020) >
Please use this identifier to cite or link to this item:
http://ir.fmu.ac.jp/dspace/handle/123456789/1354
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| Title: | Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma |
| Authors: | Kariya, Yoshinobu
Oyama, Midori
Ohtsuka, Mikio
Kikuchi, Nobuyuki
Hashimoto, Yasuhiro
Yamamoto, Toshiyuki |
| Affiliation: | 生化学講座
皮膚科学講座 |
| Source title: | Fukushima Journal of Medical Science |
| Volume: | 66 |
| Issue: | 3 |
| Start page: | 119 |
| End page: | 123 |
| Issue Date: | 2020 |
| Abstract: | α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression. |
| Publisher: | The Fukushima Society of Medical Science |
| Publisher (Alternative foam): | 福島医学会 |
| language: | eng |
| URI: | http://ir.fmu.ac.jp/dspace/handle/123456789/1354 |
| Full text URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/1354/1/FksmJMedSci_66_p119.pdf |
| ISSN: | 0016-2590
2185-4610 |
| DOI: | 10.5387/fms.2020-12 |
| PubMed ID: | 32779579 |
| Related Page: | https://doi.org/10.5387/fms.2020-12 |
| Rights: | © 2020 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license. |
| Rights: | https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| Appears in Collections: | Vol.66 (2020)
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