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Vol.66 (2020) >

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Title: Validation of MALDI-TOF MS devices in reanalysis of unidentified pathogenic bacteria detected in blood cultures
Authors: Imai, Minako
Kimura, Yukio
Tanno, Daiki
Saito, Kyoichi
Honda, Mutsuko
Takano, Yukiko
Ohashi, Kazutaka
Toyokawa, Masahiro
Ohana, Noboru
Yamadera, Yukio
Shimura, Hiroki
Affiliation: 検査部
Source title: Fukushima Journal of Medical Science
Volume: 66
Issue: 2
Start page: 103
End page: 112
Issue Date: 2020
Abstract: In hospital microbial laboratories, morphological and biochemical analyses are performed to identify pathogenic microbes;however, these procedures lack rapidity and accuracy. Recently, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been clinically utilized, and is expected to enable rapid and accurate microbial identification. We aimed to validate two MALDI-TOF MS devices available in Japan: the VITEK-MS (BioMérieux) and the Microflex LT (Bruker Daltonics). Clinically isolated bacteria, 100 samples in all, detected in blood cultures but incompletely identified by conventional procedures, were reanalyzed using the two devices. The VITEK-MS and Microflex LT, respectively, identified 49% (49/100) and 80% (80/100) of the tested bacteria at the species level, as well as 96% (96/100) and 95% (95/100) at the genus level. Among those reidentified strains, 26% (26/100) at the species level and 88% (88/100) at the genus level were concordant with each other, though three strains were unmatched. Moreover, four bacterial strains were unable to be identified using the VITEK-MS, versus five using the Microflex LT. MALDI-TOF MS devices can provide more rapid and accurate bacterial identification than ever before;however, the characteristics of each system were slightly different;therefore, it is necessary to understand the difference in performance of MALDI-TOF MS models.
Publisher: The Fukushima Society of Medical Science
Publisher (Alternative foam): 福島医学会
language: eng
URI: http://ir.fmu.ac.jp/dspace/handle/123456789/1325
Full text URL: http://ir.fmu.ac.jp/dspace/bitstream/123456789/1325/1/FksmJMedSci_66_p103.pdf
ISSN: 0016-2590
DOI: 10.5387/fms.2020-09
PubMed ID: 32713872
Related Page: https://doi.org/10.5387/fms.2020-09
Rights: © 2020 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license.
Rights: https://creativecommons.org/licenses/by-nc-sa/4.0/
Appears in Collections:Vol.66 (2020)

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FksmJMedSci_66_p103.pdf275.44 kBAdobe PDFDownload

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