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福島県立医科大学学術成果リポジトリ = Fukushima Medical University Repository >
福島医学会 = The Fukushima Society of Medical Science >
Fukushima Journal of Medical Science >
Vol.53 (2007) >
このアイテムの引用には次の識別子を使用してください:
http://ir.fmu.ac.jp/dspace/handle/123456789/214
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| タイトル: | Breakthrough of immune self-tolerance to calreticulin induced by CpG-oligodeoxynucleotides as adjuvant |
| その他のタイトル: | Breakthrough of tolerance to calreticulin |
| 著者: | Abe, Kazumichi
Ohira, Hiromasa
Kobayashi, Hiroko
Saito, Hironobu
Takahashi, Atsushi
Rai, Tsuyoshi
Kanno, Yukiko
Monoe, Kyoko
Watanabe, Hiroshi
Irisawa, Atsushi
Sato, Yukio |
| 学内所属: | 内科学第二講座
大学健康管理センター |
| 誌名/書名: | Fukushima Journal of Medical Science |
| 巻: | 53 |
| 号: | 2 |
| 開始ページ: | 95 |
| 終了ページ: | 108 |
| 発行日: | 2007年12月 |
| 抄録: | Reportedly, bacterial DNA containing unmethylated cytosine-guanosine dinucleotide motif-containing oligodeoxynucleotides (CpG-ODNs) can induce Th1-type adjuvant effects. We produced autoantibodies and induced hepatitis in mice using extracted proteins from human hepatocytes with CpG-ODNs as adjuvant. Western blot analysis was performed of sera from immunized mice and two patients with autoimmune hepatitis (AIH). When a common band was detected, N-terminal amino acid sequencing was performed to determine its site. For detection of antibodies against the identified protein (calreticulin), ELISA was performed of sera of 50 patients with AIH: 45 with primary biliary cirrhosis (PBC), 24 with chronic hepatitis C (CH), and 24 healthy controls. Mice were immunized with calreticulin protein with CpG-ODNs as adjuvant. Several reacted bands were detected in their sera; in addition, a common band to the sera of patients with AIH was detected at 60 kDa. Subsequent N-terminal amino acid sequencing revealed that the protein was human calreticulin. ELISA showed that, of patients with AIH, PBC, and CH, 30.0% (15/50), 17.8% (8/45), and 12.5% (3/24), respectively, were positive for anti calreticulin antibodies. Splenocytes from immunized mice produced IFN-gamma after they were pulsed with calreticulin protein. Histological analyses of liver specimens taken from mice immunized with calreticulin protein together with CpG-ODNs showed spotty and focal necrosis. Immunofluorescence analysis showed increased expression of calreticulin in the liver treated with CpG-ODNs. These results suggest that a breakthrough of immune self tolerance to calreticulin is induced with CpG-ODNs as adjuvant and that calreticulin protein might be a target antigen in this model. |
| 出版者: | The Fukushima Society of Medical Science |
| 出版者（異表記）: | 福島医学会 |
| 本文の言語: | eng |
| このページのURI: | http://ir.fmu.ac.jp/dspace/handle/123456789/214 |
| 本文URL: | http://ir.fmu.ac.jp/dspace/bitstream/123456789/214/1/FksmJMedSci_53_p95.pdf |
| ISSN: | 0016-2590 |
| DOI: | 10.5387/fms.53.95 |
| PubMed番号: | 18402289 |
| 関連ページ: | https://doi.org/10.5387/fms.53.95 |
| 権利情報: | © 2007 The Fukushima Society of Medical Science |
| 出現コレクション: | Vol.53 (2007)
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